Cesium Chloride Splash to Eye Due to Ill-Fitting Safety Glasses

What Happened?

A researcher was preparing a cesium chloride gradient for DNA purification. As she was eliminating bubbles in a small 5-mL vial by tapping the vial, a drop from the vial splashed into her left eye. The researcher was wearing contact lenses along with her safety glasses; however, the safety glasses were too loose. The researcher immediately rinsed her eyes at the eyewash station for 15 min, and subsequently applied a saline solution from the lab’s first aid kit. The researcher was sent to Occupational Health and Safety for medical evaluation.

What Was The Cause?

The tube containing the chemical was tabbed too harshly and liquid spilled out. Accidents like this can happen easily, however, the ill-fitting safety glasses did not offer adequate protection.

What Corrective Actions Were Taken?

  • The lab must provide a choice of eye protection for all lab members to find a good fit

How Can Incidents Like This Be Prevented?

  • Wear properly fitting eye protection



Splash of Chemical to Eyes:

  1. Immediately flush eyes at the nearest eyewash station for at least 15 minutes
  2. Get medical attention if needed
  3. Report the incident to your supervisor


Cesium Chloride 

Hazardous to skin and eyes (irritant)