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Lysis Buffer Splashed into Researcher’s Eye

What Happened?

While sorting microfuge tubes containing samples in cell lysis buffer, a researcher attempted to close the lid of a partially open tube. A small amount of lysis buffer splashed into her eye. The lab receives samples with lysis buffer that contain potentially hazardous chemicals. The researcher immediately flushed her eye at the eyewash station and was sent by her supervisor to Occupational Health and Safety for medical evaluation. At the time of the accident the researcher was not wearing safety glasses.

What Was The Cause?

The researcher had a mishap when attempting to close a partially closed microfuge tube; she was not wearing eye protection. 

What Corrective Actions Were Taken?

  • Eye protection must be worn when handling incoming samples

How Can Incidents Like This Be Prevented?

  • Treat all incoming samples as potentially hazardous and wear eye protection in addition to your lab coat and gloves



When lysis buffer splashes into your eyes:

  1. Immediately flush eyes at the nearest eyewash station for at least 15 minutes
  2. Get medical attention if needed
  3. Report the incident to your supervisor


Lysis Buffer

  • Typically contains a TRIS buffer and a detergent such as SDS or Triton X-100
  • May also contain protease inhibitors and lysozyme
  • Irritating to eyes and skin